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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs
doi: 10.3390/ijms22158042
Figure Lengend Snippet: Impact of IL-17A on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Article Snippet: Further, 0.5 µg/mL of
Techniques: Immunostaining, CCK-8 Assay, Software
Journal: International Journal of Molecular Sciences
Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs
doi: 10.3390/ijms22158042
Figure Lengend Snippet: Effect of IL-17F on MNs: ( A ) immunostaining of MAP-2 in IL-17F (5 ng/mL, 50 ng/mL, and 500 ng/mL) stimulated MNs; ( B ) the viability of MNs under different IL-17F treatments was measured using CCK-8 assay; ( C ) MAP-2 positive MNs counting under different IL-17F treatments; (D) the neurite length was calculated, using Neuron J in the FIJI software, under different treatment conditions in both cells, ns: no significance.
Article Snippet: Further, 0.5 µg/mL of
Techniques: Immunostaining, CCK-8 Assay, Software
Journal: International Journal of Molecular Sciences
Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs
doi: 10.3390/ijms22158042
Figure Lengend Snippet: Immunostaining of the IL-17 receptor (IL-17RA and IL-17RC) and MHCI in MNs: ( A ) immunostaining of IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP, FUS-P525L-EGFP, PBMCs, and a fibroblast: ( B ) co-staining of MAP-2 with IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP and FUS-P525L-EGFP cells; ( C ) the counting of IL-17RA positive cells in MNs; ( D ) IL-17RA positive staining in MNs; ( E ) MHCI positive staining in MNs. * p < 0.05 versus controls, ns: no significance.
Article Snippet: Further, 0.5 µg/mL of
Techniques: Immunostaining, Staining
Journal: International Journal of Molecular Sciences
Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs
doi: 10.3390/ijms22158042
Figure Lengend Snippet: Antagonizing IL17 protects from IL17-induced neurodegeneration: ( A ) IL-17A-neutralizing pretreatment for 0.5 h, 50 ng/mL IL-17A stimulated the MNs for three days, MAP-2 immunostaining was performed after the treatment; ( B ) the viability of MNs under the same treatment mentioned before was calculated using CCK-8 assay; ( C ) MAP-2 positive MNs were counted and (D) the neurite length of MNs were measuredunder different treatments; ( E ) immunostaining of MAP-2 under anti-IL-17RA/IL-17R receptor pretreatment for 0.5 h, then 50 ng/mL IL-17A-stimulated for three days; ( F ) viability of MNs; ( G ) MAP-2 positive MNs counting; (H) neurite length of MAP-2 positive MNs using the aforementioned treatment.* p < 0.05, ** p < 0.01 versus controls, ns: no significance.
Article Snippet: Further, 0.5 µg/mL of
Techniques: Immunostaining, CCK-8 Assay